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KMID : 0360220130540030490
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Journal of the Korean Ophthalmological Society
2013 Volume.54 No. 3 p.490 ~ p.495
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Effect of Ascorbic Acid Against the Oxidative Stress-Induced Cellular Senescence in Trabecular Meshwork Cells
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Kim Jae-Woo
Kang Sun-Hee
Lee Keun-Woo
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Abstract
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Purpose: To investigate the effect of L-ascorbic acid (LAA) on oxidative-stress-induced cellular senescence in trabecular meshwork (TM) cells. Methods: Primarily cultured human TM cells were exposed to 0, 10, or 100 μM hydrogen peroxide for 7 days with or without co-exposure of LAA. Cellular survival and nitrite production were assessed with MTT and Griess assays, respectively. SA- β-gal staining was performed to quantify cellular senescence. Results: Hydrogen peroxide decreased cellular survival, accompanied by decreased nitric oxide (NO) production. These decreases of cellular survival and NO production were abolished by co-exposure of 100 μM LAA. Analysis of SA-β-gal staining revealed that LAA inhibited hydrogen peroxide-induced cellular senescence by 6.8% (p < 0.05). Conclusions: LAA may have a protective effect against the oxidative-stress-induced cellular senescence in TM cells.
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KEYWORD
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Ascorbic acid, Oxidative stress, Senescence, Trabecular meshwork cells
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